Buses will replace trains on the Lilydale Line and Dublin Road will close to traffic, as crews work to remove two level crossings in Ringwood East and Croydon.
Replacement buses will operate between Ringwood and Lilydale from 8.30pm Friday 24 May until early-July.
Further changes will be in place during a week in late-June, with buses replacing trains between Blackburn, Belgrave and Lilydale from 8.30pm Tuesday 18 June to 11.30pm Friday 21 June and between Box Hill, Belgrave and Lilydale from 11.30pm Friday 21 June to the last service on Sunday 23 June.
Ringwood East Station will close from 8.30pm Friday 24 May until the new station opens in early-July.
Passengers should allow extra time when travelling and check the PTV website or mobile app for changes to their service.
Dublin Road will close to all traffic between Patterson Street and Railway Avenue from 7pm Friday 24 May until mid-June.
Motorists should allow up to 10 minutes extra for the detour via Mount Dandenong Road, the Maroondah Highway, Bedford Road, Canterbury Road and Bayswater Road.
While the road is closed, pedestrian access will be available at the crossing west of Dublin Road, and a free and accessible shuttle bus will operate via Eastfield Road between 7am and 7pm daily.
During this time, crews will work around the clock to lower the rail line into a 650m long trench under Dublin Road, demolish the old Ringwood East Station and build the new station, and complete construction of the new elevated rail bridge and road connections in Croydon.
When these works are complete in July, the Lilydale Line will become the first Melbourne rail line to be level crossing free, a year ahead of schedule.
Passengers are encouraged to visit ptv.vic.gov.au/disruptions for more information and to plan their journey.
Real-time traffic conditions are available at traffic.vicroads.vic.gov.au. The latest public transport information and network status is available at ptv.vic.gov.au or in the PTV mobile app.
